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Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

6) demonstrated that the gdh3 / gdh3 mutant exhibited a lower percentage of 13 C labelling of one to two carbon atoms in metabolites while a higher percentage of 13 C labelling for three or more carbon atoms in metabolites for most of TCA cycle intermediates and amino acids when compared to the wildtype

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and PpDHAR1 and PpGSTI1 were shown to localize in both the cytosol and chloroplasts