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Ann Surg Oncol 33(6):5348-5357, 2026

Cell viability and ROS and glutathione intracellular content of cells cultured in liquid medium were assessed by flow cytometry analysis of cells stained for 30 min in the dark with 0.025 M calcein-AM (ThermoFisher Scientific, C3099) at room temperature, 50 M Dichlorodihydrofluorescein-diacetate (DCFDA, ThermoFisher Scientific, D399) at 37 C, and 10 M monochlorobimane (ThermoFisher Scientific, M1381MP) at 37 C, respectively

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GABA acts as an inhibitory neurotransmitter in vertebrates and is present across Bacteria, Archaea, and Eukaryota kingdoms, exhibiting a diverse range of roles in various organisms (Wu and Sun