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Prior to staining, cryopreserved PBMCs were thawed in a water bath at 37 C and washed twice in thawing media (RPMI 1640 and 10% inactivated FBS) containing 1:10,000 Pierce Universal Nuclease (Thermo Scientific, Waltham, MA, USA)

In Vietnam and other Southeast Asian countries, deforestation is one of the major causes of habitat loss 17 , substantially affecting the species richness and abundance of both larvae and adults

Experimental extraction of gallic acid from brown sumac seed (Rhus coriaria) using supercritical carbon dioxide and ethanol as co-solvent: modeling and optimization