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Protein samples were mixed with 5 protein loading buffer to a final concentration of 1 and then boiled at 95 C for 10 min, resolved on 12% BisTris SDSPAGE gels and analyzed using a standard immunoblotting protocol

Anxiety-like behavior - In mice, supplementation with NAC from G12- P1 [96] or pre-mating until G16 [95] did not have an effect on PRS-induced anxiety-like behavior in the EPM at W10-14 [96] or in the OFT and EPM at P33-50 [95]

Enhancement by homocysteine of plasminogen activator inhibitor-1 gene expression and secretion from vascular endothelial and smooth muscle cells

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