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It significantly suppressed TNF-, IL-1, and IL-6 expression and inhibited polarization of macrophages to the M1 phenotype

Cell death in tumor cells was quantified by multiplex immunofluorescence (mIF) using cleaved Poly (ADP-ribose) polymerase (cPARP) with pan-cytokeratin (PanCK) for tumor mask visualization, Ki67 for context, and DAPI for nuclei