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Through high-performance liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based molecular weight identification, standard molecular weight comparison, and characteristic HPLC profiles of the [ 3 H]proline and [ 3 H]BPC157 standards (Supplementary Figure S2), we speculated the structures of these six components and designated them as M1M6 (Table 10)

Histological and immunohistochemistry analyses were also performed

Other considerations documented for tirzepatide in its FDA labeling include injection-site reactions, decreased appetite, and warnings such as the risk of thyroid C-cell tumors observed in rodents (a boxed warning) and pancreatitis and gallbladder events

and Kaleidoscope, BioRad)