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Supernatant was discarded, and the cell pellets were washed with wash buffer (10 mM Tris-HCl, pH = 8.0, 200 mM NaCl, 1 mM EDTA, 0.5 mM EGTA with protease inhibitors) and resuspended in the sonication buffer (10 mM Tris-HCl, pH = 8.0, 1 mM EDTA, 0.1% SDS with protease inhibitors)

Peroxyl radicals are more stable than both carbon-centered radicals and related alkoxyl radicals, with a half-life of several seconds at physiological temperature

In addition, PARylation has been strongly implicated in SG formation, suggesting that it might be a critical modifier for the dynamic assembly/disassembly of disease-related RNP granules, including disease-related RBPs, such as the FET family and TDP-43 129

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