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This approach was based on existing protocols for HCMV-specific IGRA with ELISA read-out 78,79 , HCMV-specific ELISpot assays 80 and on protocols for small-volume whole blood stimulation assays developed for determining SARS-CoV-2-specific T cell responses by qPCR 81,82

11.8 The road ahead: toward precision microbiome-based therapies Advances in biotechnology and bioinformatics are expected to deepen our understanding of probiotic mechanisms of action, accelerate the discovery of novel candidate organisms and prebiotic substrates, and enhance the reproducibility and comparability of both laboratory and clinical studies (536)