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Beyond T2DM, the hypothesis suggests a continuum of sulfur-dependent protein misfolding disorders, including AD, as evidenced by shared ER stress and PDI dysregulation [110]

Recent flux studies in tumor cells bearing isocitrate dehydrogenase 1 (IDH1) mutations indicate that these cells may be particularly dependent upon glutamine to fuel oxidative mitochondrial metabolism and thus may be responsive to inhibition of GLS or respiration [39,45]

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There isn't a universally agreed-upon answer, and frankly, the optimal protocol likely depends on the specific nature of the injury and the goals of the study