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These limits slow cellular response and structural change, which extends study timelines compared to soft tissue, where blood flow supports faster inflammatory and structural responses

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Biolayer interferometry NDs were immobilized on the sensor surface of amine reactive biosensors (AG2R) (fortBIO, PALL Life Science) after 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysulfoxuccinimide (EDC/NHS) activation to a final level between 1.2 and 1.8 nm depending on the NDs type using an Octet RED96 instrument (fortBIO, PALL Life Science)

Oral bacteria such as Prevotella , Fusobacterium , Porphyromonas , and Streptococcus are capable of metabolizing dietary choline into trimethylamine (TMA), which is subsequently converted to TMAO in the liver