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Pyroglutamic acid (5-oxoproline), a glutamate cyclized derivative, also presented a higher abundance in OVX rat serum/urine samples that was significantly reversed by HPL therapy (Figure 3D, metabolite determination results are shown in Supplementary Tables S10)

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Data from a single crystal were used for experiment (2) whilst data from two crystals were merged for experiments (1) and (3) (Table 1)

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