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Following NET or apoptosis induction, neutrophils were pelleted and resuspended in 1 annexin V binding buffer (eBioscience), containing annexin V (5 ng ml 1 ) and either propidium iodide (100 ng ml 1 , P4170, Sigma) or DAPI (40 nM, 422801, BioLegend)

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However, it was demonstrated that extracellular proteins can be taken up by cells through macropinocytosis, a process that involves a nonselective engulfment of the extracellular fluid phase, which gives rise to giant vesicles termed macropinosomes (Kerr & Teasdale, 2009)