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Perfusion was performed by flushing pre-warmed RPMI containing phenol red and L-glutamine through a 25- and 3/8-gauge needle placed into the aorta attached to Tygon tubing aided by the Masterflex L/S perfusion pump as described 81

These activities include antioxidant, immunostimulatory, anti-inflammatory, antiallergic, anticancer, antibacterial, and antiviral (Ferreira et al

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Due to this mechanism of action, acetylcysteine is also indicated as a specific antidote in paracetamol poisoning and in the course of treatment of cyclophosphamide-induced hemorrhagic cystitis, in the latter case, it provides the -SH groups necessary to inactivate acrolein, a toxic metabolite that affects the urinary mucosa, whilst not interfering with chemotherapy