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Description

cDNA was synthesized using Maxima First Strand cDNA synthesis kit (K1672, thermo scientific), and 1020 ng cDNA was used for qPCR on a QuantStudio 5 real-time PCR system with either TaqMan Universal Master Mix II and validated PrimeTime primer probe sets (Integrated DNA Technologies), or Maxima SYBR Green/ROX qPCR Master Mix (2) (K0221, Thermo Scientific) and designed primers