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k , PDK1-silenced HCT116 were re-expressed with PDK1 WT or K163R mutant, and cells were subjected to in vitro PIP3 binding assay

Finally, we showed that Put can be converted to GAB via a diamine oxidase (DAO)-dependent reaction as its inhibitor aminoguanidine (AG) completely blocked the production of [ 13 C 4 ]GAB from [ 13 C 4 ]Put (Fig