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However, since GHcy-SO 2 H contains a CS bond rather than an SS bond, it cannot undergo the final hydrolytic step of the catalytic cycle and instead accumulates as a dead-end product, leading to time-dependent inactivation of the enzyme ( -glutamyl transpeptidase ( FIGURE 12 TST TST, also known as rhodanese, is a mitochondrial matrix enzyme that participates in both cyanide detoxification and mitochondrial sulfide oxidation (Way, 1984)

This difference occurred by the 4th week (28 weeks) of drug administration (Figure 2C)

Alternatively, perhaps sites of ER-mitochondrial contact facilitate spatially restricted exchange of glutathione as they do for other metabolites such as calcium and reactive oxygen species (Joseph, Booth, Young, & Hajnoczky, 2019)