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Epigenetic Regulation Through Methylation 5-Amino-1MQ enables investigation of NNMTs role in methylation homeostasis: SAM/SAH Ratio: Measurement of methylation capacity through SAM and SAH quantification Global DNA Methylation: Studies on genomic methylation patterns Histone Methylation: Investigation of histone methyltransferase activity and histone marks Gene Expression: Examination of methylation-sensitive gene regulation Metabolic Reprogramming: Research on epigenetic contribution to metabolic phenotype Research addresses whether NNMT competes for SAM with other critical methyltransferases

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The resulting loss of SIRT1 impairs AMPK phosphorylation, thereby augmenting SREBP1-mediated de novo fatty acid synthesis