female rana clamitans melanota File:Rana MN.jpg North American Green Frog (Lithobates

Studies have shown that these systems effectively maintain therapeutic drug levels in the eye, enhancing treatment adherence and reducing the incidence of ocular surface disease associated with conventional therapies (243, 244)

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This was not possible for the in vitro and in vivo studies, as these experiments were performed by individual investigators who were aware of the experimental groups and treatment outcome

By contrast, both the regeneration of GSH from GSSG (recycling pathway) and de novo synthesis of GSH, by glutamate-cysteine ligase (GCL) and glutathione synthase (GS), are required to maintain intracellular GSH levels (64, 87)