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The in vitro BBB models were treated likewise

2.9 Estimation of lipid peroxidation (LPO) Lipid peroxidation was assessed by determining MDA (a thiobarbituric acid reactive species: TBARS) spectrophotometrically following the method of Beuge and Aust [11]

However, the TRIP12-dependent K29-linked ubiquitylation of OTUD5 was partially decreased by UBR5 knockdown, whereas TRIP12-dependent increase of K48-linked chains was massively diminished by UBR5 knockdown (Fig

Conversely, genetic inhibition of autophagy (e.g., Atg7 knockdown) reduced apoptosis and preserved spermatogenesis, suggesting that excessive autophagy can be harmful to germ cells 42