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Inhibition assay of cysteine cathepsin proteases The cathepsin B inhibitor screening assay 57 uses the ability of cathepsin B to cleave the synthetic AMC (7-amino-4-methylcoumarin)-based peptide substrate to release AMC, which can be quantified using a fluorometer or fluorescence microplate reader

This diagnosis was made on symptoms as my b12 was within range on the labs

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(2014) reported that at short-term, B deficiency affects cytosolic Ca 2+ levels, and in roots, upregulates the expression of genes from the MYB protein family involved in Ca 2+ signaling and represses genes of the bZIP protein family with roles as channels/transporters, sensor relays and responders that act as intermediaries in a transduction pathway triggered by B deficiency, with important consequences in plant development, growth, flower maturation and stress (Zhao et al., 2021b)