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In a recent study of 275 PD patients and 144 controls, as well as atypical PD cohorts, neuronal EVs were isolated using L1CAM immunocapture, followed by mass spectrometry or electrochemiluminescence to measure proteins including alpha-synuclein and clusterin 106 : Compared with controls and other neurodegenerative conditions including MSA, levels of mean neuron-derived exosomal alpha-synuclein were two-fold higher in clinical PD and prodromal PD, with an AUC of 0.86 for separating patients with PD from controls 106

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Intracellular fluorescence was then observed using an inverted fluorescence microscope