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The Tipping Point The problem arises when you take large doses of acetaminophen or use it very frequently

10.1155/2021/1470380 92 LiuP.LiJ.LiuM.ZhangM.XueY.ZhangY.et al (2021c)

followed by 40 cycles of denaturation at 95 C for 5 s and annealing-extension at 60C for 30 s (two-step method)