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All the reagents and the chemicals which were used in this study were obtained from Sigma Chemicals Co., USA

doi: 10.3389/fneur.2026.1779076 Received 31 December 2025 Revised 03 March 2026 Accepted 16 March 2026 Published 07 April 2026 Volume 17 - 2026 Edited by Ming-Chyi Pai, National Cheng Kung University, Taiwan Reviewed by Ye Lv, Capital Medical University, China Vasiliki Moraiti, MindOT, Greece Updates Copyright 2026 Bednorz and Religa

The ability to detect late-onset responses makes the model particularly appropriate for evaluation of chemical allergy, where late-onset responses are a frequent occurrence

2 Moisture, Crude protein (CP), crude fiber (CF), ether extract (EE), calcium (Ca) and phosphorus (P) were measured and recorded