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First, the chemical identification was performed once, with the methodology of the 1970s, and was not subsequently re-verified by modern peptide sequencing a point Kovalzon and Strekalova later flagged as a source of doubt about whether the isolated nonapeptide was a single defined entity or a mischaracterized fraction of a larger precursor (Kovalzon & Strekalova, 2006)

The tripeptide sequence glycyl-L-histidyl-L-lysine wraps around a copper ion, creating a structure with remarkable stability when handled correctly and surprising fragility when handled wrong