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2.3 Preparation of sperm suspensions On February 24 th , 2024, frogs were euthanised according to methods approved by the University of Wollongong s Animal Ethics Committee (see section 2.8 Ethics review statement below), and testis tissue immediately extracted, weighed, and macerated in 170L of simplified amphibian ringer (SAR: 113mM NaCl, 1mM CaCl 2 , 2mM KCl, 3.6mM NaHCO 3 )

As can be seen in Figure 6D, yeast transformed with plasmid-encoded gliT were capable of growth in the presence of gliotoxin (16 and 64 g/ml, respectively) depending on whether minimal or rich media was used to support growth, while those transformed with empty vector were unable to grow, irrespective of what media conditions were used

Luft, J