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Liu Y, Hyde AS, Simpson MA, Barycki JJ: Emerging regulatory paradigms in glutathione metabolism

Antioxidant enzymes and peroxidation in liver tissue To assess oxidative damage and the effects of the test drug, liver tissue samples were homogenized in 0.1 M sodium phosphate buffer (pH 7.4) containing phenylmethylsulfonyl fluoride (PMSF) and then centrifuged at 4,000 g for 10 min at 5C

Ask them to write their predictions as to what will happen before dropping the ice cube and then note their observations of how the water moves and changes colour