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Interaction materials [edit | edit source] These are typical biochemical interactions in nature that have been used extensively in affinity chromatography: Enzyme will bind to substrate analogue, inhibitor, and cofactor Lectin will bind to polysaccharide, glycoprotein, cell surface receptor, cell Antibody will bind to antigen, virus, cell Nucleic acid will attach to complementary base sequence, histones, nucleic acid polymerase, and nucleic acid binding protein Hormone, vitamin will bind to receptor, carrier protein Glutathione will bind to glutathione-S-transferase or GST fusion proteins Metal ions will attach to Poly (His) fusion proteins, native proteins with histidine, cysteine and tryptophan residues on their surfaces