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Long chain polyunsaturated fatty acids (LC-PUFA), notably 20:4(n-6) arachidonic (ARA), 20:5(n-3) eicosapentaenoic (EPA), and 22:6(n-3) docosahexaenoic (DHA) acids, as well as one of the two essential fatty acids, 18:3(n-3) -linolenic acid (ALA) are some of the least abundant, although a wide inter-individual variation exists that is dependent on maternal diet and genetics (Table 1) (50)

Hypoxic conditions (0.1% O 2 ) were reached by automatically pumping N 2 gas into the plate reader chamber controlled by the ACU system

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4.8 Regulating cellular autophagy function Cellular autophagy is a metabolic regulatory process that recycles and degrades damaged organelles, proteins, and other biomolecules, thereby supporting the growth and development of organisms (Wang et al., 2024)