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The sections were washed before being incubated with secondary antibodies at 4C overnight accordingly and then with ExtrAvidin (Sigma-Aldrich Pty, Ltd., Sydney, AU) at room temperature for 3 hours after being washed, developed in 3, 3, diaminobenzidine tetrahydrochoride (DAB, Sigma-Aldrich Pty, Ltd.) and cover slipped with DPX