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Getting the Dose Right More is not automatically better

Step 9: Snap Freezing Snap freeze aliquots in liquid nitrogen or dry ice/ethanol bath before transferring to -80C storage

99,219 In excitatory neurons, CaMKII functions as a shuttle molecule to collect Ca 2+ /Calmodulin from the cytoplasm and transport it to the nucleus, where Ca 2+ /Calmodulin release activates CaMKK and its substrate CaMKIV to further phosphorylate CREB, thereby participating in the regulation of transcription and translation