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While this study suggested that non-denatured whey protein isolate may be safe and may have acceptable tolerability in children with autism or ASD, the primary endpoint examined was limited to tolerability

In particular, DNA samples (50 ng) were amplified using the respective LightSNiP Assay for each SNP and Lightcycler FastStart DNA Master HybProbe Mix (Roche, Germany), according to the following PCR protocol: initial denaturation for 10 min at 95C, followed by 45 cycles of denaturation for 10 s at 95C, primer annealing for 10 s at 60C and extension for 15 s at 72C, followed by melting curve analysis to determine homozygosity for the wild-type alleles, heterozygosity, and homozygosity for the variant alleles

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Clearly, some kind of selectivity must be exercised if complex mixtures are to be avoided