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The results showed that the fluorescence intensity at the tumor site gradually increased over time, reaching a peak at 6 h and maintaining a relatively high intensity for the subsequent 42 h, indicating the rapid and prolonged accumulation of nanoparticles at the tumor site (Fig

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Sections were blocked with 3% BSA (30 min, room temperature), then incubated with a primary antibody against GSDMD (ab209845) overnight at 4 C