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Cells were washed twice and incubated with LPS (100 ng/mL) in XF based medium (Agilent, 103334-100) supplemented with 4.5 g/L glucose, 2 mM glutamine, 1 mM sodium pyruvate, and 1 mM HEPES buffer at final pH7.4 for 3 hr

doi: 10.1038/s41574-023-00845-0

Melanie Avalon: I've actually thought about this before and I don't know if this is going to sound controversial to you, because people will say, "Drink with food because you won't get the effects as much." Basically, when I'm drinking, I actually like to have a glass on an empty stomach, because I get a bigger effect with less alcohol [giggles] rather than if I had more alcohol, which maybe I shouldn't be saying this

There is no mixing, reconstitution, or injection it is an oral small-molecule compound, not an injectable peptide