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Near infrared secondary antibody and CellTag700 total protein stain were added at desired concentration (Table S1) to 1.5% BSA and incubated with fixed cell culture overnight at 4 C

plots concatenated from one experiment (nave, MCMV and vCD44BP, n = 4/group) and representative of four independent experiments

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coli expression vectors can contain a protease recognition sequence between the fusion partner coding gene and the passenger protein coding gene that allows the tag removal when the latter protein is for using in protein therapies, vaccine development and structural analyses