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The worm pellet was stained with 5 M Cell ROX Deep Red for 1 h

Some research studies have used higher doses for specific conditions, but high doses should only be taken under the supervision of a healthcare provider

Model building and refinement The structure model of apo-form ABCC2 was built based on an initial model predicted by AlphaFold2 52 and refined with secondary structure and geometry restraints, automatically or manually using Real-space refinement in PHENIX 1.20.1 53 or WinCoot 0.9.8.1 54

Endoglin, an ancillary TGF receptor, is required for extraembryonic angiogenesis and plays a key role in heart development