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3 It has been estimated that glaucoma is the second cause of blindness in humans and that 68 million patients suffer from this pathology, about seven million individuals having bilateral blindness

Polymerization occurred at 37 C for 30 min, culture medium was overlaid and image capture performed as previously described (protocols.io For Boyden chamber invasion assays, cells were incubated in 0.1% inactivated BSA for 24 h and then plated in Matrigel-covered Boyden chambers (6 g/96-well, bottom treated with collagen type I) and incubated for 8 h at 37 C and 5% CO 2 atmosphere, with 5 ng/mL of epithelial growth factor as a chemoattractant

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Monitor patients closely for signs of drug interactions and adjust the dosage of concomitant medications as necessary