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Biomarker validation The Receiver Operating Characteristic (ROC) curve analysis was performed using MetaboAnalyst 6.0 online tool/ Biomarker analysis

The limit of quantification and limit of detection of Lid were also determined, with the limit of quantification calculated as 2.046 g/mL and the limit of detection as 0.675 g/mL

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The difference in the effects of SLC protein expression on tumor cells and immune cells suggests that targeting the metabolism of tumor cells, inhibiting glutamine uptake by tumor cells, reversing the dominant position of glutamine competition in tumor cells in the TME, and activating immune function to kill tumor cells may be new strategies to explore