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The mechanisms include reduction of excitotoxicity and oxidative stress, enhanced neurotrophic factor expression supporting neuronal survival, angiogenesis improving blood flow, and modulation of dopamine and serotonin systems

Ethical principles of the study complied with the European Directive 010/63/E, the Law on Amendments to the Animal Protection Act (Official Gazette 37/13), the Animal Protection Act (Official Gazette 135/06), the ordinance on the protection of animals used for scientific purposes (Official Gazette 55/13), Federation of European Laboratory Animal Science Associations (FELASA) recommendations, and the recommendations of the Ethics Committee of the School of Medicine, University of Zagreb

Each 0.1 mL (10 units on an insulin syringe) would then deliver 0.5 mg

Group 2 (control + AST) was treated with AST (100 mg/kg, i.p) for 14 days