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Transfection was carried out for 24 h with Lipofectamine 3000 (L3000075, Invitrogen, USA) and P3000 (L3000075, Invitrogen, USA) transfection reagents mixed with OPTI-MEM (Gibco, Shanghai, China)

There was a significant average paired fold change in the levels of several GPs 1 week after 4-week intermittent fasting compared with the levels before 4-week intermittent fasting (Supplementary Table S2)

(2) Documentation must include the specific muscles injected, units used per muscle, and the Modified Ashworth Scale score justifying treatment

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