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Description

1987;253:H17683

doi: 10.1590/s0004-2803.201700000-18 46 KamathB

Method development & assays: solvent for compatibility, stability and assay-development work requiring a preserved diluent

As the ChaC1p and ChaC2p enzymatic assays were coupled assays involving the cys-gly dipeptidase enzyme Dug1p (coupled to ChaC1p or ChaC2p), it was possible that the compound juglone could be inhibiting this second enzyme and not ChaC1 or ChaC2