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Cells were maintained in Dulbeccos Minimal Eagle Medium (DMEM) with 10% fetal bovine serum (FBS), Blasticidin, and Penicillin/Streptomycin and then transferred to 1.5 ml freezing vials and stored under liquid nitrogen in growth media containing 5% DMSO

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Drugs such as capecitabine, methotrexate, and various tyrosine kinase inhibitors require intact renal or hepatic pathways for clearance

For researchers exploring alternatives to gavage, encapsulation remains the most reliable method to balance stress reduction, dose certainty, and taste elimination, provided the delayed absorption window is compatible with behavioral testing schedules