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The cell pellets were centrifuged for 10 min at 600 g and 4 C, resuspended in fluorescence-activated cell sorting (FACS) buffer (PBS with 0.5% bovine serum albumin (BSA) and 5 mM EDTA), and filtered through a 70 m nylon mesh filter (Biosharp, Beijing, China

These antivirulence molecules target various bacterial mechanisms, including direct neutralization of virulence factors, inhibition of their secretion, interfering with bacterial signaling, preventing biofilm formation, reversal of resistance, and suppressing virulence factor expression [87, 88]