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Acknowledgements The authors are thankful to all participants who have volunteered in this study and also laboratory of Physiology department of Guilan University staff for technical analysis

Diabetic painful neuropathy has been shown to be mediated via an inflammatory chemokine pathway (23) and strategies to reduce this inflammation, including gene transfer of soluble TNF receptor, have been shown to alleviate pain (24)

Therefore, it is crucial to develop innovative antidepressant treatments that are based on a thorough understanding of its etiological mechanisms

34 A derivatization procedure was used using phthalaldehyde