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D., & Cavanaugh, J

Vitamin D3: Vitamin D3 plays an enormous role in the development and function of the brain

Int Immunopharmacol 146:113801

8 mM of reducing agent DTT (Sigma) was then added to the samples and incubated in a thermomixer at 30C for 30 minutes, followed by the addition of 20 mM iodoacetamide (Sigma) for protein carboxylation with incubation at 30C in the dark for 30 minutes