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This transient, noise-induced paradoxical phenomenon, which precedes the eventual silencing of auditory nerve fibers and synapses, may be characterized by (1) excessive glutamate release from IHCs, resulting in overactivation of receptors on auditory nerve fibers 66 , and (2) functional compensation by LOC efferent neurons, ensuring that auditory nerve activity is enhanced during periods of increased demand 67 , consistent with evidence suggesting that a primary function of LOC efferents is to reduce auditory neuropathy 68

Conversely, H 2 O 2 /O have been shown to impair antioxidant enzymes function, thereby diminishing the cell's defenses against oxidative damage and amplifying oxidative stress [100]

N74pAcF, predicted to have the most significant improvement in binding affinity, indeed showed enhanced binding compared to the wild type