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Description

In this example, the oval and rectangle domains are only in the flow-through, but not in the elution so they do not interact with your protein of interest

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c) To confirm SILAC data, SKOV3 cells, seeded on T25 flasks, were transfected with plasmids pTagRFP-C-SRSF4 and pEGFP-C1-TIA1 131 using Lipofectamine 3000 (Invitrogen) according to the manufacturers protocol

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