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Description

In vitro-differentiated embryonic stem cells give rise to male gametes that can generate offspring mice

All fluorescence images were imaged by an UltraView Vox Spinning Disk Confocal Microscope (PerkinElmer) (63X)

Microscopy SEM was done with a SUPRA 40VP (Carl Zeiss MicroImaging) equipped with a K1250X Cryogenic SEM Preparation System (EMITECH), a CPD 030 critical point dryer (Bal-Tec) and a SC 7600 sputter coater (Polaron) at the on-campus microscopy core facility Zentrale Mikroskopie (CeMic) of the Max Planck Institute for Plant Breeding Research, Cologne