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After overnight incubation, cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilised with 0.2% TritonX-100, blocked with 5% of goat serum and incubated overnight at 4 C with the anti-mature IGF-1 antibody (Prepotech)

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The released AA can then be converted into other chemical mediators, such as various prostaglandins (PGs) and platelet activating factors (PAF), via cyclooxygenases (COXs) and lipoxygenases (LOXs) to induce an inflammatory response

Recently, an oxygen-independent supramolecular photodynamic agent was developed to oxidize water to generate highly cytotoxic hydroxyl radicals directly