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The majority of commercially available biotechnological methods for pest management on crops involve the production of Bt insecticidal crystalline proteins from Bacillus thuringiensis , referred as Cry toxins, which aid in the permeabilization of the gut epithelial cell membrane in susceptible insects (Vaughan et al

Moreover, pharmacokinetic constraints-including limited bioavailability and uncertain brain penetration-must be considered when interpreting gene-expression effects observed in vitro