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46 In patients with CHB, HBV-specific CD8 + T cells were verified to have an increased propensity to express CTLA-4 strongly correlating with viral load

Cell culture Swiss 3T3 fibroblasts, obtained from American Type Culture Collection (ATCC CCL 92), were maintained at 37 in Dulbecco's modified Eagle's medium supplemented with 25 mM HEPES (pH 7.4), 10% (v/v) fetal bovine serum, 100 units/mL penicillin, and 100 mg/mL streptomycin

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